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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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rat il 4 elisa kit  (RayBiotech inc)
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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dupilumab recombinant human mab anti il4 and il13  (Sanofi)
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels of IL-4 (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.

Journal: Frontiers in Physiology

Article Title: Interleukin-13 R130Q aggravates airway smooth-muscle phenotypic switching and promotes airway remodeling in severe asthma

doi: 10.3389/fphys.2026.1906184

Figure Lengend Snippet: Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels of IL-4 (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.

Article Snippet: According to the instructions of the manufacturer for the IL-4 and IL-5 ELISA kits (RayBiotech, Peachtree Corners, GA, USA), 100 μL of each sample was added to pre-coated wells and incubated at room temperature for 2.5 h. The wells were then washed three times for 5 min each, and blank wells served as negative controls.

Techniques: Expressing

Effects of IL-13 130R and IL-13 130Q on human bronchial smooth muscle cell (hBSMC) phenotypes (n = 4 per group). (A) CCK-8-derived proliferative/metabolic activity. (B) Representative Fluo-3/AM flow-cytometry histograms and median fluorescence intensity quantification of the acetylcholine-induced intracellular calcium response. (C, D) IL-5 (C) and IL-4 (D) concentrations in culture supernatants. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; SEM, standard error of the mean.

Journal: Frontiers in Physiology

Article Title: Interleukin-13 R130Q aggravates airway smooth-muscle phenotypic switching and promotes airway remodeling in severe asthma

doi: 10.3389/fphys.2026.1906184

Figure Lengend Snippet: Effects of IL-13 130R and IL-13 130Q on human bronchial smooth muscle cell (hBSMC) phenotypes (n = 4 per group). (A) CCK-8-derived proliferative/metabolic activity. (B) Representative Fluo-3/AM flow-cytometry histograms and median fluorescence intensity quantification of the acetylcholine-induced intracellular calcium response. (C, D) IL-5 (C) and IL-4 (D) concentrations in culture supernatants. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; SEM, standard error of the mean.

Article Snippet: According to the instructions of the manufacturer for the IL-4 and IL-5 ELISA kits (RayBiotech, Peachtree Corners, GA, USA), 100 μL of each sample was added to pre-coated wells and incubated at room temperature for 2.5 h. The wells were then washed three times for 5 min each, and blank wells served as negative controls.

Techniques: CCK-8 Assay, Derivative Assay, Activity Assay, Flow Cytometry, Fluorescence